The complete amino acid sequence of antiviral protein from the seeds of pokeweed (Phytolacca americana).

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Complete amino acid sequence of chitinase-A from leaves of pokeweed (Phytolacca americana).

The complete amino acid sequence of pokeweed leaf chitinase-A was determined. First all 11 tryptic peptides from the reduced and S-carboxymethylated form of the enzyme were sequenced. Then the same form of the enzyme was cleaved with cyanogen bromide, giving three fragments. The fragments were digested with chymotrypsin or Staphylococcus aureus V8 protease. Last, the 11 tryptic peptides were pu...

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Purification and partial characterization of another form of the antiviral protein from the seeds of Phytolacca americana L. (pokeweed).

1. The pokeweed antiviral protein, previously identified in two forms (PAP and PAP II) in the leaves of Phytolacca americana (pokeweed) [Obrig. Irvin & Hardesty (1973) Arch. Biochem. Biophys. 155, 278-289; Irvin, Kelly & Robertus (1980) Arch. Biochem. Biophys. 200, 418-425] is a protein that prevents replication of several viruses and inactivates ribosomes, thus inhibiting protein synthesis. 2....

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The Complete Amino Acid Sequence of ( Pltytolacca americana ) Chitinase - Bfrom the Leayes of Pokeweed

The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 try, ptic peptides deriyed from the redu ¢ ed and S-carboxy, methylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecu...

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The Complete Amino Acid Sequence of ( Pltytolacca americana ) Chitinase - Bfrom

The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 try, ptic peptides deriyed from the redu ¢ ed and S-carboxy, methylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecu...

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ژورنال

عنوان ژورنال: Agricultural and Biological Chemistry

سال: 1990

ISSN: 0002-1369,1881-1280

DOI: 10.1271/bbb1961.54.3301